As climate change-induced fluctuations in extreme precipitation events are expected to grow, the consequences on arbuscular mycorrhizal fungi (AMF) community composition are becoming increasingly relevant, but remain understudied. The overarching goal of my study is to determine how fluctuations in soil moisture alter AMF community dynamics and, in turn, modulate the growth response of Viola sagittata, a conservative prairie violet and key host to an endangered butterfly (Regal fritillary). My research includes both field studies of seasonal shifts in soil moisture and AMF communities in Viola in remnant and restored prairies, and a greenhouse study of AMF-inoculated Viola seedling growth and survival under simulated drought. Together, these studies will inform the extent to which potential AMF partners are tolerant of soil drying, and whether they alleviate soil moisture stress in the host Viola. 

The REU student will participate in all field, lab and greenhouse components of the study to gain experiences in soil collection and chemical analyses, fungal DNA extraction, root staining and microscopy, and monitoring plant performance in the greenhouse. At the same time, we expect that the REU intern will pursue their own research project related to the project. No prior experience in soil or AMF analyses is expected.